Our labs perform western blotting in house. We have the expertise to detect relative protein expression as well as total protein expression using our setup. The image to the left shows a PVDF membrane with GAPDH positive bands alongside a molecular ladder. The isolate is extracted from a hippocampal rat culture cell line.
We have expertise in designing, performing and interpreting ELISAs. Currently focused on in-house R&D of a line of synthetic combination passivating/chemically activating coatings known collectively as ISurBlock. ISurBlock solution can act both as a non-animal sourced BSA substitute and as a platform for chemically bonding biomacromolecules that are components of the ELISA assay.
We do routine PCR experiments to detect gene expression. Our labs are equipped with thermal cyclers as well as equipment for performing gel electrophoresis in-house. The image to the left shows the results of a mycoplasma screen on an agarose gel. The PCR products are from human mesenchymal stem cells.
At ISurTec, we perform microbiological assays on our coated surfaces. We test for adherence of microbes to the surface as well as microbicidal effects of drug release. The image to the left is a Kirby-Bauer zone of inhibition test demonstrating a bacteria-free zone from moxifloxacin release in the upper section of a Mueller-Hinton plate with E. coli.