ISurTherm is a proprietary thermoresponsive coating available on many cell culture substrates. The use of these surfaces replaces the use of trypsin for collecting cells since detachment is achieved merely by lowering the culture temperature to ambient room temperature. This eliminates the need for trypsin; hence saving on washing, labor, and time.
The thermoresponsive T25 ISurTherm® flasks from ISurTec® allow users to harvest strongly adherent cells without enzymatic digestion. The harvested cells retain high viability and intact cell junction proteins. Without the need for trypsin or other enzymes for passaging, cost and contamination risk are greatly reduced. ISurTherm is designed for use with strongly adherent cell lines such as human mesenchymal stem cells (hMSC) as well as typical adherent cell types, including fibroblast and endothelial cells.
Applications: ISurTherm is ideally suited for the collection of viable cells with intact cell surface proteins and gap junctions without the use of digestive enzymes like trypsin or other harsh treatment.
Cells cultured for 48 hrs on an ISurTherm plate and released at room temperature after 5 minutes (left) before forming a cell sheet after 20 minutes (right).
At room temperature, cell sheets will spontaneously release within 30 minutes