Pipeline Products

ISurCellULA Surfaces

Ultra-Low Attachment Cell Cultureware for Spheroid Production

ISurCell cultureware are ultra-low attachment (ULA) plates that promote spheroid formation. They are available in different formats— 6-well, 12-well, 24-well, 96-well and cell culture flasks. The ISurCell ULA plates are coated with our proprietary ultra-low attachment coating on standard polystyrene. ISurCell ULA coatings can be adapted for other cell culture surfaces upon request.

Applications: ISurCell ULA plates can be used for a wide variety of applications such as creating tissue models for drug discovery and development, suspension cultures, and organoid technology.

Beta Testing
Brain organoids stained with FOXG1, a telencephalic marker cultured on a 96-well ISurCell plate

Pipeline Products

ISurCellULA Surfaces

Ultra-Low Attachment Cell Cultureware for Spheroid Production

ISurCell cultureware are ultra-low attachment (ULA) plates that promote spheroid formation. They are available in different formats— 6-well, 12-well, 24-well, 96-well and cell culture flasks. The ISurCell ULA plates are coated with our proprietary ultra-low attachment coating on standard polystyrene. ISurCell ULA coatings can be adapted for other cell culture surfaces upon request.

Applications: ISurCell ULA plates can be used for a wide variety of applications such as creating tissue models for drug discovery and development, suspension cultures, and organoid technology.

Brain organoids stained with FOXG1, a telencephalic marker cultured on a 96-well ISurCell plate
Beta Testing

ISurCell ULA Cultureware

As it prevents biomolecule adsorption, ISurCell prevents cell adherence, promoting spheroid formation

ISurCell kits consists of coated cell cultureware available in different formats. Upon seeding with cells, ISurCell ULA plates spontaneously promote spheroid formation and suspension culture. ISurCell can be applied to other cell cultureware formats upon request.

Human dermal fibroblast spheroids on ISurCell 96-well plate

Human mesenchymal stem cells on 24-well ISurCell plate

ISurCell plates in 24-well and 96-well format

LIVE/DEAD assay done on cells cultured on ISurCell ULA cultureware shows no cytotoxicity.

LIVE/DEAD staining assay on human dermal fibroblast cells

Green denotes live cells stained with Calcein AM, while Red stain denotes dead cells stained with Sytox orange (Magnification 10x)

Bright phase image of human dermal fibroblasts cells on ISurCell ULA plate

(Magnification 4x)

ISurCell ULA plates can be used for differentiating cells and tissues. We have successfully differentiated iPSC- derived human neural progenitor cells to cortical spheroids on our plates using neural differentiation media and growth factors. These spheroids express FOXG1, a cortical/telencephalic marker. Spheroids are viable for more than 2 months with no cytotoxicity, and they show neurite extension.

Free floating cortical spheroids on 96-well ISurCell ULA plates

Cortical spheroids with neurites extending 6 weeks into differentiation

Cortical spheroids with neurites extending at 2 months into differentiation

Cells seeded at high density on competitor plate and on ISurCell.

ISurCell ULA Plate

Single Spheroid

Larger Field of View

Competitor Plate

Multiple Spheroids

Narrow Field of View

Neural progenitor cells at 4x total magnification seeded at a density of 100,000 cells/well. ISurCell ULA plates reduce cell satellite clusters and multiple spheroid formation.

Spheroids are not attached to the plates and can be easily transferred to other wells even after 2 months of culturing.

ISurCell ULA Cultureware

As it prevents biomolecule adsorption, ISurCell prevents cell adherence, promoting spheroid formation

ISurCell kits consists of coated cell cultureware available in different formats. Upon seeding with cells, ISurCell ULA plates spontaneously promote spheroid formation and suspension culture. ISurCell can be applied to other cell cultureware formats upon request.

Human dermal fibroblast spheroids on ISurCell 96-well plate

Human mesenchymal stem cells on 24-well ISurCell plate

ISurCell plates in 24-well and 96-well format

LIVE/DEAD assay done on cells cultured on ISurCell ULA cultureware shows no cytotoxicity.

LIVE/DEAD staining assay on human dermal fibroblast cells

Green denotes live cells stained with Calcein AM, while Red stain denotes dead cells stained with Sytox orange (Magnification 10x)

Bright phase image of human dermal fibroblasts cells on ISurCell ULA plate

(Magnification 4x)

ISurCell ULA plates can be used for differentiating cells and tissues. We have successfully differentiated iPSC- derived human neural progenitor cells to cortical spheroids on our plates using neural differentiation media and growth factors. These spheroids express FOXG1, a cortical/telencephalic marker. Spheroids are viable for more than 2 months with no cytotoxicity, and they show neurite extension.

Free floating cortical spheroids on 96-well ISurCell ULA plates

Cortical spheroids with neurites extending 6 weeks into differentiation

Cortical spheroids with neurites extending at 2 months into differentiation

Cells seeded at high density on competitor plate and on ISurCell.

ISurCell ULA Plate

Single Spheroid

Larger Field of View

Competitor Plate

Multiple Spheroids

Narrow Field of View

Neural progenitor cells at 4x total magnification seeded at a density of 100,000 cells/well. ISurCell ULA plates reduce cell satellite clusters and multiple spheroid formation.

Spheroids are not attached to the plates and can be easily transferred to other wells even after 2 months of culturing.

EXPLORE R&D

EXPLORE R&D

ISurTec is a technology innovator with a deep passion for identifying, creating and commercializing new methods and products that enable companies to fulfill their mission.

CONTACT

Innovative Surface Technologies, Inc.
1045 Westgate Drive Suite 100
Saint Paul, MN 55114

651-209-9757

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ISurTec will consistently provide high-quality products and services that meet requirements for scientific, medical device, and industrial coatings. We will actively strive to bring excellence to the surface in all that we do, meeting or exceeding customers’ needs and expectations, and improve the quality of our products and service by enabling each employee to fulfill their job responsibilities correctly the first time.
The following are trademarks of ISurTec: ISurTec®, ISurGlide®, ISurLite®, ISurTherm®, ISurCoat®, PhotoPrime®, ISurGuard™, ISurBlock™, ISurCell™. All rights are expressly reserved.