ISurTherm is a proprietary thermoresponsive coating that achieves detachment by lowering the culture temperature to ambient room temperature. This eliminates the need for trypsin; hence saving on washing, labor, and time.
The thermoresponsive T25 ISurTherm® flasks from ISurTec® allow users to harvest strongly adherent cells without enzymatic digestion. The harvested cells retain high viability and intact cell junction proteins. Without the need for trypsin or other enzymes for passaging, cost and contamination risk are greatly reduced. ISurTherm is designed for use with strongly adherent cell lines such as human mesenchymal stem cells (hMSC), as well as typical adherent cell types, including fibroblast and endothelial cells
Cells cultured for 48 hrs on an ISurTherm plate and released at room temperature after 5 minutes (left) before forming a cell sheet after 20 minutes (right).
At room temperature, cell sheets will spontaneously release within 30 minutes
ISurTherm works on multiple cell lines, showing an advantage in release with most lines over current commercial products.
Release measured after 48 hrs of culturing, quantified using the ViaLight ATP-detection assay according to standard protocol, by an independent laboratory.
Improved mesenchymal stem cell release over commercial products: Complete detachment of mesenchymal stem cells (MSCs) from ISurTherm surfaces occurred within 30 min at room temperature, as compared with no significant MSC detachment on Product A surface.
ISurTec is a technology innovator with a deep passion for identifying, creating and commercializing new methods and products that enable companies to fulfill their mission.
Innovative Surface Technologies, Inc.
1045 Westgate Drive Suite 100
Saint Paul, MN 55114
651-209-9757
ISurTec will consistently provide high quality products and services that meet requirements for medical device coatings and other scientific coatings. We will actively strive to bring excellence to the surface in all that we do, meeting or exceeding customers’ needs and expectations, and improve the quality of our products and service by enabling each employee to fulfill their job responsibilities correctly the first time.
The following are trademarks of ISurTec: ISurTec®, ISurGlide®, ISurLite®, ISurTherm®, ISurCoat®, PhotoPrime®, ISurGuard™, ISurBlock™, ISurCell™. All rights are expressly reserved.