ISurTherm® Cultureware

ISurTherm Thermoresponsive Cell
Culture Surfaces

ISurTherm is a proprietary thermoresponsive coating that achieves detachment by lowering the culture temperature to ambient room temperature. This eliminates the need for trypsin; hence saving on washing, labor, and time.

The thermoresponsive T25 ISurTherm® flasks from ISurTec® allow users to harvest strongly adherent cells without enzymatic digestion. The harvested cells retain high viability and intact cell junction proteins. Without the need for trypsin or other enzymes for passaging, cost and contamination risk are greatly reduced. ISurTherm is designed for use with strongly adherent cell lines such as human mesenchymal stem cells (hMSC), as well as typical adherent cell types, including fibroblast and endothelial cells

Applications

ISurTherm is ideally suited for the collection of viable cells with intact cell surface proteins and gap junctions without the use of digestive enzymes like trypsin or other harsh treatment.

Cells cultured for 48 hrs on an ISurTherm plate and released at room temperature after 5 minutes (left) before forming a cell sheet after 20 minutes (right).

At room temperature, cell sheets will spontaneously release within 30 minutes

ISurTHerm Cultureware

ISurTherm Functionality

ISurTherm is available on different culture surfaces and can be optimized for cell type adherence strengths.
The coating is compatible and non-cytotoxic with multiple cell lines, including mesenchymal stem cells, offering an advantage over current commercial products.

Advantages

ISurTherm works on multiple cell lines, showing an advantage in release with most lines over current commercial products.

Release measured after 48 hrs of culturing, quantified using the ViaLight ATP-detection assay according to standard protocol, by an independent laboratory.

Improved mesenchymal stem cell release over commercial products: Complete detachment of mesenchymal stem cells (MSCs) from ISurTherm surfaces occurred within 30 min at room temperature, as compared with no significant MSC detachment on Product A surface.

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We look forward to collaborating with you to accelerate your success.