Pipeline Products

ISurTherm® Cultureware

Thermoresponsive cell cultureware for non-enzymatic cell sheet release

ISurTherm is a proprietary thermoresponsive coating available on many cell culture substrates. The use of these surfaces replaces the use of trypsin for collecting cells since detachment is achieved merely by lowering the culture temperature to ambient room temperature. This eliminates the need for trypsin; hence saving on washing, labor, and time.

Applications: ISurTherm is ideally suited for the collection of viable cells with intact cell surface proteins and gap junctions without the use of digestive enzymes like trypsin or other harsh treatment.

Beta Testing
(Click to enlarge images)

Cells cultured for 48 hrs on an ISurTherm plate and released at room temperature after 5 minutes (left) before forming a cell sheet after 20 minutes (right).

At room temperature, cell sheets will spontaneously release within 30 minutes

Pipeline Products

ISurTherm® Cultureware

Thermoresponsive cell cultureware for non-enzymatic cell sheet release

ISurTherm is a proprietary thermoresponsive coating available on many cell culture substrates. The use of these surfaces replaces the use of trypsin for collecting cells since detachment is achieved merely by lowering the culture temperature to ambient room temperature. This eliminates the need for trypsin; hence saving on washing, labor, and time.

Applications: ISurTherm is ideally suited for the collection of viable cells with intact cell surface proteins and gap junctions without the use of digestive enzymes like trypsin or other harsh treatment.

(Click to enlarge images)

Cells cultured for 48 hrs on an ISurTherm plate and released at room temperature after 5 minutes (left) before forming a cell sheet after 20 minutes (right).

At room temperature, cell sheets will spontaneously release within 30 minutes

Beta Testing
ISurTherm cell sheet release stained with Connexin-43

ISurTherm Functionality

ISurTherm is available on different culture surfaces and can be optimized for cell type adherence strengths. The coating is compatible and non-cytotoxic with multiple cell lines, including mesenchymal stem cells, offering an advantage over current commercial products.  

ISurTherm Cultureware Includes:

  • Flasks
  • Multiwell Plates
  • Petri dishes
  • Film inserts

ISurTherm works on multiple cell lines, showing an advantage in release with most lines over current commercial products.

Release measured after 48 hrs of culturing, quantified using the ViaLight ATP-detection assay according to standard protocol, by an independent laboratory.

Improved mesenchymal stem cell release over commercial products:

Complete detachment of mesenchymal stem cells (MSCs) from ISurTherm surfaces occurred within 30 min at room temperature as compared with no significant MSC detachment on the Product A surfaces.

Cell gap junctions, surface proteins, and extracellular matrix proteins are preserved as no chemical or protease treatment is required for release.

A) LIVE/DEAD cytotoxicity shows the coating is non-toxic to the cells and B) Connexin-43 (CX-43 a gap junction marker) staining on a cell sheet that has released from the surface shows the majority are CX-43 immunoreactive.

ISurTherm has also been applied to nanofibers and microcarriers for custom work:

A) LIVE/ DEAD cytotoxicity assay of BAE cells growing on ISurTherm-coated polystyrene microcarriers
B) High power (400x magnification) of BAE cells growing on ISurTherm-treated microcarriers
C) Low temperature release of BAE cells from the microcarriers
D) Released cell suspension after 20 min. at room temperature
E) Released cells growing on cell culture surfaces after reseeding (75 cm2 flasks)

ISurTherm Functionality

ISurTherm cell sheet release stained with Connexin-43

ISurTherm is available on different culture surfaces and can be optimized for cell type adherence strengths. The coating is compatible and non-cytotoxic with multiple cell lines, including mesenchymal stem cells, offering an advantage over current commercial products.  

ISurTherm Cultureware Includes:

  • Flasks
  • Multiwell Plates
  • Petri dishes
  • Film inserts

ISurTherm works on multiple cell lines, showing an advantage in release with most lines over current commercial products.

Release measured after 48 hrs of culturing, quantified using the ViaLight ATP-detection assay according to standard protocol, by an independent laboratory.

Improved mesenchymal stem cell release over commercial products:

Complete detachment of mesenchymal stem cells (MSCs) from ISurTherm surfaces occurred within 30 min at room temperature as compared with no significant MSC detachment on the Product A surfaces.

Cell gap junctions, surface proteins, and extracellular matrix proteins are preserved as no chemical or protease treatment is required for release.

A) LIVE/DEAD cytotoxicity shows the coating is non-toxic to the cells and B) Connexin-43 (CX-43 a gap junction marker) staining on a cell sheet that has released from the surface shows the majority are CX-43 immunoreactive.

ISurTherm has also been applied to nanofibers and microcarriers for custom work:

A) LIVE/ DEAD cytotoxicity assay of BAE cells growing on ISurTherm-coated polystyrene microcarriers
B) High power (400x magnification) of BAE cells growing on ISurTherm-treated microcarriers
C) Low temperature release of BAE cells from the microcarriers
D) Released cell suspension after 20 min. at room temperature
E) Released cells growing on cell culture surfaces after reseeding (75 cm2 flasks)

EXPLORE R&D

EXPLORE R&D

ISurTec is a technology innovator with a deep passion for identifying, creating and commercializing new methods and products that enable companies to fulfill their mission.

CONTACT

Innovative Surface Technologies, Inc.
1045 Westgate Drive Suite 100
Saint Paul, MN 55114

651-209-9757

LEARN MORE

ISurTec will consistently provide high-quality products and services that meet requirements for scientific, medical device, and industrial coatings. We will actively strive to bring excellence to the surface in all that we do, meeting or exceeding customers’ needs and expectations, and improve the quality of our products and service by enabling each employee to fulfill their job responsibilities correctly the first time.
The following are trademarks of ISurTec: ISurTec®, ISurGlide®, ISurLite®, ISurTherm®, ISurCoat®, PhotoPrime®, ISurGuard™, ISurBlock™, ISurCell™. All rights are expressly reserved.