ISurBlock solution can be applied as a short blocking step to vials. This simple application prevents subsequent adsorption of proteins from solution to container walls. Here, 1.5-ml polypropylene microvials received 0.25 ml ISurBlock solution (or BSA, or blank PBS), were tumbled at RT for 1 hr, washed x3 with PBS with vortexing, and then tumbled again with a solution of peroxidase
The treatments left to right are: BSA, ISurBlock sample 1 and ISurBlock sample 2, plain PBS.* The strong color development in the uncoated vial on the far right is due to adsorbed peroxidase, and the reduction of color in the others is a reflection of their respective treatments’ ability to prevent such protein adsorption.
*The vials were exposed to HRP solution for 1hr at room temp after the various treatments.
ISurBlock significantly reduces unwanted protein adsorption to exposed surfaces.